erik dent (Addgene inc)

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Addgene inc erik dent
Erik Dent, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/erik+dent/Double+UP+Halotag+to+mScarlet+(Plasmid+%23125138)/pm40157353-300-94-99
Average 91 stars, based on 5 article reviews

erik dent - by Bioz Stars, 2026-09

91/100 stars

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Plasmid Preparation:

Article Title: Microtubule depolymerization contributes to spontaneous neurotransmitter release in vitro
Article Snippet: Culture medium was DMEM/F12 [1:1] containing 2.5% fetal bovine serum, 2.5% rat serum (prepared in the animal care facility of the Campus of Bellvitge, University of Barcelona), 5 nM NGF (Alomone Labs, Jerusalem, Israel, N-100), 2 nM CNTF (Alomone Labs, C-245), and 25 U/ml penicillin/streptomycin at 37 °C and 8% CO 2 . .. EB3-tdTomato was a gift from Erik Dent (Addgene plasmid # 50708; http://n2t.net/addgene:50708 ; RRID:Addgene_50708) . .. The coding sequence of EB3-tdTomato was cloned into pWPXL (Addgene plasmid # 12257; http://n2t.net/addgene:12257 ; RRID:Addgene_12257)) by replacing EGFP using MluI-HF and NdeI.

Article Title: CDKL5’s role in microtubule-based transport and cognitive function
Article Snippet: .. The following plasmids were used for transfection: EB3-tdTomato was a gift from Erik Dent (Addgene plasmid #50708), pTRIP-CAG-hTTL-eGFP and pTRIP-CAG-eGFP were a gift from Carsten Janke, HA-BICD2N-FRB-PCI-NEO and GW1-PEX-mRFP-FKBP were gifts from Lukas Kapitein, Lamp1-RFP and pCl-fusionred-GluR2(R) were a gift from Daniel Choquet. ..

Article Title: Centrosome-dependent microtubule modifications set the conditions for axon formation
Article Snippet: .. EB3-tdTomato was a gift from Erik Dent (Addgene plasmid # 50708; http://n2t.net/addgene:50708 ; RRID:Addgene_50708 ( )), pBrain-GFP-shTACC3 was a gift from Stephen Royle (Addgene plasmid # 59355; http://n2t.net/addgene:59355 ; RRID: Addgene_59355 ( )), pBrain-GFP-shGL2 was a gift from Stephen Royle (Addgene plasmid # 60004; http://n2t.net/addgene:60004 ; RRID:Addgene_60004 ( )). ..

Article Title: Centrosome-dependent microtubule modifications set the conditions for axon formation.
Article Snippet: .. EB3-tdTomato was a gift from Erik Dent (Addgene plasmid # 50708; http://n2t.net/addgene:50708; RRID:Addgene_50708 (Merriam et al., 2013)), pBrain-GFP-shTACC3 was a gift from Stephen Royle (Addgene plasmid # 59355; http://n2t.net/addgene:59355; RRID: Addgene_59355 (Booth et al., 2011)), pBrain-GFP-shGL2 was a gift from Stephen Royle (Addgene plasmid # 60004; http://n2t.net/ addgene:60004; RRID:Addgene_60004 (Booth et al., 2011)). ..

Article Title: Microtubule depolymerization contributes to spontaneous neurotransmitter release in vitro.
Article Snippet: Culture medium was DMEM/F12 [1:1] containing 2.5% fetal bovine serum, 2.5% rat serum (prepared in the animal care facility of the Campus of Bellvitge, University of Barcelona), 5 nM NGF (Alomone Labs, Jerusalem, Israel, N-100), 2 nM CNTF (Alomone Labs, C-245), and 25 U/ml penicillin/streptomycin at 37 °C and 8% CO2. .. EB3-tdTomato was a gift from Erik Dent (Addgene plasmid # 50708; http://n2t. net/addgene:50708; RRID:Addgene_50708)18. .. The coding sequence of EB3tdTomato was cloned into pWPXL (Addgene plasmid # 12257; http://n2t.net/ addgene:12257; RRID:Addgene_12257)) by replacing EGFP using MluI-HF and NdeI.

Transfection:

other:

Article Title: Animal septins contain functional transmembrane domains.
Article Snippet: This work N/A C. elegans strain MDX152; genotype: knuSi934 [pNU3400 (AMAD01- unc-61p:: wrmScarlet::UNC-61(B/C):: unc-61u, unc-119(+))] II; unc-119(ed)III; unc-61a (mon26 [unc-61a L7_V29del]) V. This work N/A Oligonucleotides Primers for mammalian transgenes, See Table S1 This work N/A Primers to confirm MosSCI animals, See Table S2 This work N/A crRNA, ssDNA donor, confirmatory primers for CRISPR-edited C. elegans, See Table S3 This work N/A Recombinant DNA cDNA: HsSEPT10_i1 Gift from Andrew Wilde (University of Toronto) N/A Gene fragment: msfGFP::CsSEPT10C Eurofins Genomics N/A Gene fragment: HsSEPT10_i1DC-CsSEPT10C Eurofins Genomics N/A Plasmid: Double UP Halotag to mScarlet Gift from Erik Dent (University of Wisconsin-Madison) Addgene plasmid #125138 Plasmid: mScarlet3 Gift from Dorus Gadella (University of Amsterdam) Addgene plasmid #189753 Plasmid: UNC-61a TMD1-48:HaloTag This work pJAP9 Plasmid: msfGFP::HsSEPT10_i1 This work pM321 Plasmid: msfGFP::CsSEPT10C This work pM324 Plasmid: msfGFP::HsSEPT10_i1DC-CsSEPT10C This work pM325 Plasmid: mScarlet3-CAAX This work pM300 Plasmid: UNC-61a TMD1-48::msfGFP This work pJAP11 Plasmid: TOMM20::mApple Gift from Michael Davidson (Florida State University) Addgene plasmid #54955 Plasmid: GalT::mCherry Gift from Michael Davidson (Florida State University) Addgene plasmid #55052 Plasmid: HaloTag::Sec61b Gift from Christopher Obara (UCSD) N/A Software and algorithms UniProt Uniprot Consortium29 https://uniport.org Interactive Tree of Life Letunic and Bork57 https://itol.embl.de (Continued on next page) ll Current Biology 35, 1910–1917.e1–e5, April 21, 2025 e2 Report

Recombinant:

Article Title: Centrosome-dependent microtubule modifications set the conditions for axon formation.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-acetylated Tubulin Sigma Cat# T7451; RRID:AB_609894 Rat anti-a-Tubulin SySy Cat# 302217; RRID:AB_314247 Mouse anti-Ankyrin G (463) Santa Cruz Cat# sc-12719; RRID:AB_626674 Mouse anti-Arl13b BioLegend Cat# N295B/66; RRID:AB_2877361 Guinea pig anti-bIII-Tubulin SySy Cat# 302304; RRID:AB_10805138 Rabbit anti-Cep120 this manuscript N/A Mouse anti-GAPDH Santa Cruz Cat# sc-32233; RRID:AB_627679 Rabbit anti-Pericentrin Covance Cat# PRB-432C; RRID:AB_2313709 Mouse anti-polyglutamylated Tubulin Sigma Cat# T9822; RRID:AB_477598 Mouse anti-SMI 31 BioLegend Cat# 801601; RRID:AB_2564641 Rabbit anti-TACC3 Cell Signaling Cat# 8069; RRID:AB_10830219 Mouse anti-Tau-1 Millipore Cat# MAB3420; RRID:AB_11213630 Rat anti-tryosinated Tubulin (YL1/2) Abcam Cat# ab6160; RRID:AB_305328 Sheep anti-Tubulin (total) Cytoskeleton Cat# ATN02; RRID:AB_10708807 anti-guinea pig goat IgG AF647 Invitrogen Cat# A21450; RRID:AB_141882 anti-mouse goat IgG HRP Dianova Cat# 115-035-003: AB_10015289 anti-mouse donkey IgG AF 488 Invitrogen Cat# A21202; RRID:AB_141607 anti-mouse goat IgG STAR 580 Abberior Cat# 2-0002-500-1; RRID:AB_228307 anti-mouse donkey IgG AF 647 Invitrogen Cat# A31571; RRID:AB_162542 anti-rabbit goat IgG AF 488 Invitrogen Cat# A11077; ; RRID:AB_2534121 anti-rabbit goat IgG HRP Dianova Cat# 111-035-003; RRID: AB_2313567 anti-rabbit donkey IgG AF 647 Invitrogen Cat# A31573; RRID:AB_2536183 anti-rat donkey IgG HRP Dianova Cat# 712-035-153; RRID:AB_2340639 anti-rat goat IgG AF 568 Invitrogen Cat# A11077; RRID:AB_2534121 anti-rat goat IgG STAR RED Abberior Cat# STRED-1007-500UG anti-sheep donkey IgG HRP Dianova Cat# 713-035-147; RRID:AB_2340710 anti-sheep donkey IgG STAR RED Abberior Cat# STRED-1056-500UG Chemicals, peptides, and recombinant proteins Hoechst dye Invitrogen Cat# 33258; RRID:AB_2651133 Cytochalasin D Sigma Cat# C2618-200UL Taxol Sigma Cat# T7402 Nocodazole Sigma Cat# M1404-10MG Spindlactone B Axon Medchem Cat# 2474 TMT10plexTM Reagent Set for Isobar Marking Thermo Scientific Cat# 90110 Deposited data Data related to Figures 2, 3, 6, and S7 Mendeley data https://doi.org/10.17632/cvk3nf2mpr.1 Experimental models: Cell lines BL21-CodonPlus (De3)-RIL competent cells Agilent Technologies cat# 230280 Experimental models: Organisms/strains Mice; C57BL6J Jackson https://www.jax.org Rats; HsdCpb:WU (all rat cultures otherwise mentioned) Envigo https://www.envigo.com (Continued on next page) e1 Cell Reports 39, 110686, April 19, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Rats; HanRj:WI (Figures 3A and 3B) Janvier https://www.janvier-labs.com Recombinant DNA CAG-Venus a gift from Zhigang Xie; de Anda et al., 2010 N/A pSilencer2-U6-shControl Xie et al., (2007) N/A pSilencer2-U6-shCep120 Xie et al., (2007) N/A pAAV-CAG-tDimer a gift from Thomas Oertner N/A CMV-EB3-tdTomato a gift from Erik Dent (via Addgene); Merriam et al., (2013) RRID:Addgene_50708 CMV- Actin-mCherry a gift from Pirta Hotulainen; Bertling et al., (2016) N/A pBrain-GFP-TACC3KDP-shTACC3 a gift from Stephen Royle (via Addgene); Booth et al., (2011) RRID:Addgene_59356 pBrain-GFP-shGL2 a gift from Stephen Royle (via Addgene); Booth et al., (2011) RRID:Addgene_60004 pBrain-GFP-shTACC3 a gift from Stephen Royle (via Addgene); Booth et al., (2011) RRID:Addgene_59355 CMV-GFP-TACC3 a gift from Stephen Royle (via Addgene); Booth et al., (2011) RRID:Addgene_59356 CMV-tdTomato-TACC3 This manuscript N/A CAG-Farnesylated-GFP a gift from Annette Gärtner; de Anda et al., 2010 N/A pNeuroD-GFP a gift from Zhigang Xie; de Anda et al., 2010 N/A pET-21a Novagen Cat# 69740-3 Software and algorithms Fiji Max-Planck-Gesellschaft http://fiji.sc/; RRID: SCR_002285 ImageJ NIH https://imagej.net/; RRID:SCR_003070 Graphpad Prism versions 8 and 9 GraphStats https://www.graphpad.com/; RRID: SCR_002798 Adobe Illustrator Adobe https://www.adobe.com/products/ illustrator.html; RRID: SCR_014198 Huygens Professional Scientific Volume Imaging RRID: SCR_014237 ..

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erik dent (Addgene inc)

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